Sedimentation Coefficients of Yeast Ribosomes

Sedimentation coefficients of RNA from 70S and 80S ribosomes.

It has been shown' previously that the corrected sedimentation coefficients (820 W) of the ribosomes from 25 species of fungi and 25 species of bacteria, respectively, range from 76.5 to 84.5 Svedberg units (S) and from 63.8 to 72.1S, and that the difference between the arithmetic means, 81.3 and 68.4S, of these two frequency distributions is significant. Since the ribosomes from other eucaryot...

Magnesium binding to yeast ribosomes.

Pressure-induced dissociation of sedimenting ribosomes: effect on sedimentation patterns.

Anomalous sedimentation patterns arise when free ribosomes from sea urchin eggs are centrifuged at high speeds. Pressure-induced dissociation of the ribosomes during sedimentation can explain the peculiar behavior; the assumption of such dissociation also yields estimates of the equilibrium constant (as a function of KCl concentration) and the change in molecular volume (500 +/- 100 ml/mol) in ...

Characterization of mitochondrial ribosomes from yeast.

A method for the preparation of mitochondrial ribosomes from yeast is presented. Mitochondrial ribosomes differ from their cytoplasmic counterpart in nucleotide composition, electrophoretic mobility of a number of their respective ribosomal proteins, and thermal denaturation profiles. The mitochondrial and cytoplasmic ribosomes have identical sedimentation coefficients (80 S) and dissociate int...

The assembly of ribosomes in yeast.

The ribosomal proteins of the yeast Saccharomyces cerevisiae have been examined by gel electrophoresis. The large subunit has about 50 proteins, with molecular weights ranging from 10,000 to 46,000; the small subunit has about 30 proteins, with none larger than 30,000. Newly formed ribosomal protein appears on ribosomes during a period of about 20 min after its synthesis. At this time much of t...